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KMID : 1007520010100050572
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Food Science and Biotechnology
2001 Volume.10 No. 5 p.572 ~ p.575
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Detection of Irradiated Chicken Wing and Thigh Using the DNA Comet Assay
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SONG, KYUNG BIN
Lee, Seung Cheol/Yang, Jae Seung
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Abstract
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Ionizing radiation can be used to prolong the shelf life of the meat products by reducing the number of pathogenic bacteria. To elucidate DNA damage caused by irradiation, DNA comet assay was used for analyzing chicken thighs and wings irradiated at 1, 5, and 10 kGy. With increasing radiation dose, the length of comet tails increased. Above 5 kGy, the tails were completely separated from the heads. At 10 kGy, the separated tails showed clouds of several short DNA fragments. Distribution pattern of the tail lengths of the comets clearly suggests that DNA comet assay could be a rapid and inexpensive method for the detection of fresh irradiated chicken.
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